STEROIDOGENIC EFFECTS OF KOREAN RICE BRAN EXTRACT ON MOUSE NORMAL LEYDIG CELLS VIA MODULATION OF STEROIDOGENESIS-RELATED ENZYMES
1Department of Urology, Chung-Ang University College of Medicine, Seoul, Republic of Korea
2Bio-Integration Research Center for Nutra-Pharmaceutical Epigenetics, Chung-Ang University, Seoul, Republic of Korea
DOI: 10.31083/jomh.v16i4.282 Vol.16,Issue 4,October 2020 pp.45-53
Published: 01 October 2020
Background and objective
Many plant extracts have various effects on diseases. In particular, some medicinal plants have been shown to have steroidogenic effects in men. However, the effects of Korean rice bran extract (RBE) on steroidogenesis in men have not been reported previously. Accordingly, in this study, we investigated the effects of RBE (Oryza sativa L.) on mouse normal Leydig cells (TM3), which produce testosterone with increasing levels of key enzymes (e.g., StAR, CYP11A1, and CYP17A1) for male steroidogenesis.
Material and methods
Using mouse normal Leydig cells (TM3), we determined whether RBE had steroidogenic effects by eval-uating changes in the levels of steroidogenesis-related enzymes, such as StAR (steroidogenic acute regu-latory protein), CYP11A1, and CYP17A1, using quantitative real-time PCR, western blotting analysis, and ELISA method.
RBE significantly enhanced mRNA and protein levels of StAR, CYP11A1, and CYP17A1, thereby enhanc-ing synthetic testosterone levels in mouse Leydig TM3 cell supernatants. These findings indicated that RBE increased the levels of steroidogenic enzymes to modulate steroidogenesis.
RBE enhanced mRNA and protein levels of key enzymes including StAR, CYP11A1, and CYP17A1 for male steroidogenesis. These changes could induce testosterone production by TM3 cells in vitro. Thus, RBE may be used as a food additive or medicinal plant for the treatment of diseases characterized by insuf-ficient testosterone (e.g., late-onset hypogonadism).
Korean rice bran extract; Oryza sativa L.; medicinal plant; steroidogenesis; late-onset hypogonadism
Hyun Joo Chung,Jin-Wook Kim,Chae-Eun Lee,Soon Chul Myung. STEROIDOGENIC EFFECTS OF KOREAN RICE BRAN EXTRACT ON MOUSE NORMAL LEYDIG CELLS VIA MODULATION OF STEROIDOGENESIS-RELATED ENZYMES. Journal of Men's Health. 2020. 16(4);45-53.
1. Miller WL. Molecular biology of steroid hormone synthesis. Endocr Rev 1988;9:295–318. https://doi. org/10.1210/edrv-9-3-295
2. Beattie MC, Adekola L, Papadopoulos V, et al. Leydig cell aging and hypogonadism. Exp Gerontol 2015;68:87–91. https://doi.org/10.1016/j. exger.2015.02.014
3. Stocco DM, Clark BJ. Regulation of the acute pro-duction of steroids in steroidogenic cells. Endocr Rev 1996;17:221–44. https://doi.org/10.1210/er.17.3.221
4. Stocco DM. StAR protein and the regulation of steroid hormone biosynthesis. Annu Rev Physiol 2001;63:193–213. https://doi.org/10.1146/annurev. physiol.63.1.193
5. Culty M, Luo L, Yao ZX, et al. Cholesterol trans-port, peripheral benzodiazepine receptor, and steroidogenesis in aging leydig cells. J Androl 2002;23:439–47.
6. Decaroli MC, Rochira V. Aging and sex hormones in males. Virulence 2017;8:545–70. https://doi.org/1 0.1080/21505594.2016.1259053
7. Grober ED. Testosterone deficiency and replace-ment: Myths and realities. Can Urol Assoc J 2014;8(7–8 Suppl 5):S145–7. https://doi.org/10.5489/cuaj.2309
8. Chung HJ, Noh Y, Kim MS, et al. Steroidogenic effects of Taraxacum officinale extract on the lev-els of steroidogenic enzymes in mouse leydig cells. Animal Cells Syst 2018;22:407–14. https://doi.org/1 0.1080/19768354.2018.1494628
9. Martillanes S, Rocha-Pimienta J, Gil MV, et al. Antioxidant and antimicrobial evaluation of rice bran (Oryza sativa L.) extracts in a mayonnaise-type emulsion. Food Chem 2020;308:125633. https://doi. org/10.1016/j.foodchem.2019.125633
10. Um MY, Um MY, Yang H, et al. Rice bran extract supplement improves sleep efficiency and sleep onset in adults with sleep disturbance: A random-ized, double-blind, placebo-controlled, polysom-nographic study. Sci Rep 2019;9:12339. https://doi. org/10.1038/s41598-019-48743-8
11. Sukhonthara S, Kaewka K, Theerakulkait C. Inhibitory effect of rice bran extracts and its phe-nolic compounds on polyphenol oxidase activ-ity and browning in potato and apple puree. Food Chem 2016;190:922–7. https://doi.org/10.1016/j. foodchem.2015.06.016
12. Xiao J, Zhang R, Wu Y, et al. Rice bran phenolic extract protects against alcoholic liver injury in mice by alleviating intestinal microbiota dysbiosis, barrier dysfunction, and liver inflammation medi-ated by the endotoxin-TLR4-NF-kappaB pathway. J Agric Food Chem 2020;68:1237–47. https://doi. org/10.1021/acs.jafc.9b04961
13. Martillanes S, Ayuso-Yuste MC, Gil MV, et al. Bioavailability, composition and functional char-acterization of extracts from Oryza sativa L. bran. Food Res Int 2018;111:299–305. https://doi. org/10.1016/j.foodres.2018.05.057
14. Wang W, Guo J, Zhang J, et al. Isolation, identifi-cation and antioxidant activity of bound pheno-lic compounds present in rice bran. Food Chem 2015;171:40–9. https://doi.org/10.1016/j.foodchem. 2014.08.095
15. Kondo S, Teongtip R, Srichana D, et al. Antimicrobial activity of rice bran extracts for diarrheal disease. J Med Assoc Thai 2011;94(Suppl 7):S117–21.
16. Ray B, Hutterer C, Bandyopadhyay SS, et al. Chemically engineered sulfated glucans from rice bran exert strong antiviral activity at the stage of viral entry. J Nat Prod 2013;76:2180–8. https://doi. org/10.1021/np4003977
17. Akihisa T, Yasukawa K, Yamaura M, Ukiya M, et al. Triterpene alcohol and sterol ferulates from rice bran and their anti-inflammatory effects. J Agric Food Chem 2000;48:2313–19. https://doi. org/10.1021/jf000135o
18. Luo L, Chen H, Zirkin BR. Are leydig cell ste-roidogenic enzymes differentially regulated with aging? J Androl 1996;17:509–15.
19. Luo L, Chen H, Zirkin BR. Leydig cell aging: Steroidogenic acute regulatory protein (StAR) and cholesterol side-chain cleavage enzyme. J Androl 2001;22:149–56.
20. Luo L, Chen H, Zirkin BR. Temporal relationships among testosterone production, steroidogenic acute regulatory protein (StAR), and P450 side-chain cleavage enzyme (P450scc) during Leydig cell aging. J Androl 2005;26:25–31.
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