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Original Research

Open Access


  • Jinhee Woo1,†
  • Kwangha Hwang1,†
  • Yul-Hyo Lee3
  • Hee-Tae Roh1

1Department of Physical Education, College of Arts and Physical Education, Dong-A University, Busan, Korea

2Laboratory of Exercise Physiology, Department of Physical Education, Graduate School, Dong-A University, Busan, Korea

3Department of Taekwondo, Youngsan University, Yangsan-si, Korea

DOI: 10.31083/jomh.v16i4.281 Vol.16,Issue 4,October 2020 pp.133-140

Published: 01 October 2020

*Corresponding Author(s): Hee-Tae Roh E-mail:

† These authors contributed equally.

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Background and objective

The purpose of this study was to investigate the effects of regular exercise training on nicotinamide ade-nine dinucleotide/sirtuin 1 (NAD+/SIRT1) signaling protein levels in skeletal muscles of middle-aged and old-aged mice.

Material and methods

Experimental animals were 40 male C57BL/6 mice out of which 20 were 38-week-old (middle-aged) and the other 20 were 58-week-old (aged). They were divided into four groups: middle-aged control (MC), mid-dle-aged exercise (ME), aged control (AC), and aged exercise (AE) groups (n = 10, each group). ME and AE groups performed exercise training five times weekly for 8 weeks using animal treadmill, after which gastrocnemius muscles were excised and analyzed.


After 8 weeks of intervention, protein levels of AMP-activated protein kinase (AMPK), SIRT1, forkhead box protein 1 (FOXO1), and NAD+ levels were significantly lower in AC group than in MC group (p < 0.05). In addition, AMPK, SIRT1, FOXO1, NAD+, and peroxisome proliferator-activated receptor gamma coact-ivator 1-alpha (PGC-1α) levels were significantly higher in ME and AE groups that exercised for 8 weeks than in MC and AC groups that did not exercise (p < 0.05).


These results suggest that aging and exercise training have opposite effects on the NAD+/SIRT1 pathway in gastrocnemius muscles and that exercise training can be effective in up-regulation of the aging-related NAD+/SIRT1 pathway.


aging; AMPK; exercise training; FOXO1; NAD+; PGC-1α; SIRT1

Cite and Share

Jinhee Woo,Kwangha Hwang,Yul-Hyo Lee,Hee-Tae Roh. EFFECTS OF EXERCISE TRAINING ON AGING-RELATED NAD+/SIRT1 PATHWAY IN MIDDLE-AGED AND AGED MICE. Journal of Men's Health. 2020. 16(4);133-140.


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