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Original Research

Open Access Special Issue

Effects of aerobic exercise training on circulating angiopoietin-like protein 2 in overweight and obese men: a pilot study

  • Jiyeon Park1
  • Keisei Kosaki1
  • Kanae Myoenzono2,3
  • Youngju Choi1,4
  • Takehiko Tsujimoto5
  • Nobutake Shimojo6
  • Seiji Maeda1,7

1Faculty of Health and Sport Sciences, University of Tsukuba, Tsukuba, 305-8574 Ibaraki, Japan

2Graduates School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, 305-8574 Ibaraki, Japan

3Humanome Lab., Inc., Chuo-ku, 104-0045 Tokyo, Japan

4Institute of Sports and Arts Convergence (ISAC), Inha University, 22212 Incheon, Republic of Korea

5Faculty of Human Sciences, Shimane University, 690-8504 Shimane, Japan

6Emergency and Critical Care Medicine, Faculty of Medicine, University of Tsukuba, 305-8575 Tsukuba, Japan

7Faculty of Sport Sciences, Waseda University, 359-1192 Saitama, Japan

DOI: 10.31083/jomh.2021.121

Submitted: 22 July 2021 Accepted: 02 September 2021

Online publish date: 17 September 2021

*Corresponding Author(s): Seiji Maeda E-mail:

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Background and objective: Angiopoietin-like protein 2 (ANGPTL2) is a pro-inflammatory adipokine that is upregulated in obesity and plays a role in the progression of cardiometabolic diseases, including diabetes and atherosclerosis. Aerobic exercise is one of the effective strategies for reducing the levels of various pro-inflammatory biomolecules in obese individuals. However, the effects of aerobic exercise training on circulating ANGPTL2 levels in obese individuals remain unclear. The objective of this study was to investigate the effect of aerobic exercise training on serum ANGPTL2 levels in overweight and obese men.

Material and methods: Twenty overweight and obese men (age, 49 ± 10 years; body mass index, 27.4± 2.2 kg/m2) completed a 12-week aerobic exercise training program (60–85% Heart ratemax, 40–60 min/day, 3 days/week). Before and after the exercise program, serum ANGPTL2 levels were measured using the enzyme-linked immunosorbent assay. Daily step counts and the different physical activities based on the intensity were assessed using a triaxial accelerometer.

Results: Serum ANGPTL2 levels were significantly decreased after the 12-week aerobic exercise training program ((3.0 ± 0.6) vs. (2.7 ± 0.7) ng/mL, P < 0.05). Daily step counts ((8362 ± 4551) vs.(10357 ± 3168) steps/day, P < 0.05) and moderate- to vigorous-intensity physical activity (MVPA) time ((58 ± 45) vs. (76 ± 37) min/day, P < 0.001) were significantly increased after the exercise intervention. The changes in serum ANGPTL2 levels were negatively correlated with corresponding changes in daily step counts (partial r = –0.49, P < 0.05) and MVPA time (partial r = –0.47, P < 0.05) after adjustment for age and accelerometer wear time.

Conlcusion: These findings collectively suggest that aerobic exercise training, in particular an increase in MVPA, can be associated with decreased circulating levels of ANGPTL2 in overweight and obese men.


Angiopoietin-like protein 2; Inflammation, Aerobic exercise; Obesity

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Jiyeon Park,Keisei Kosaki,Kanae Myoenzono,Youngju Choi,Takehiko Tsujimoto,Nobutake Shimojo,Seiji Maeda. Effects of aerobic exercise training on circulating angiopoietin-like protein 2 in overweight and obese men: a pilot study. Journal of Men's Health. 2021.doi:10.31083/jomh.2021.121.


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