Article Data

  • Views 449
  • Dowloads 135

Original Research

Open Access

ATF3 inhibits the growth and stem cells-like features of SW620 colorectal cancer cells in vitro

  • Chuanqian Huang1
  • Changdan Chen2
  • Fangjing Zheng1
  • Xiaoxiao Ni1
  • Jianfang Lin1
  • Wenjuan Wu1
  • Xiaolan Lai3

1Department of Medical Oncology and Radiotherapy, Ningde Municipal Hospital Affiliated to Ningde Normal University, 352000 Ningde, China

2Department of Gastroenterology, Ningde Municipal Hospital Affiliated to Ningde Normal University, 352000 Ningde, China

3Department of Hematology and Rheumatism, Ningde Municipal Hospital Affiliated to Ningde Normal University, 352000 Ningde, China

DOI: 10.31083/jomh.2021.072 Vol.17,Issue 4,September 2021 pp.287-294

Submitted: 06 April 2021 Accepted: 08 June 2021

Published: 30 September 2021

*Corresponding Author(s): Xiaolan Lai E-mail: drxiaolan@163.com

PDF (6.76 MB)

Abstract

Background and objective: Activating transcription factor 3 (ATF3) plays a crucial role in regulating tumor development depending on the cellular context or cancer cell type. However, the effect of ATF3 on stem cells-like features in colorectal cancer (CRC) has yet to be elaborated.

Methods: In this study, we overexpressed ATF3 in SW620 CRC cells to investigate its effects on stem cells-like features.

Results: Our results indicated that overexpressing ATF3 inhibited the proliferation, invasion, migration, and sphere formation capacity of SW620 CRC cells. ATF3 overexpression also decreased the size of tumorspheres and reduced expression of the cancer stem cell markers CD44 and CD133 in SW620 cells.

Conclusion: In summary, our study revealed that ATF3 suppresses CRC growth and stem cells-like features. ATF3 is considered a potential target in CRC therapy.

Keywords

ATF3; Migration; Invasion; Cancer stem cells

Cite and Share

Chuanqian Huang,Changdan Chen,Fangjing Zheng,Xiaoxiao Ni,Jianfang Lin,Wenjuan Wu,Xiaolan Lai. ATF3 inhibits the growth and stem cells-like features of SW620 colorectal cancer cells in vitro. Journal of Men's Health. 2021. 17(4);287-294.

References

[1] Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians. 2018; 68: 394–424.

[2] Wrobel P, Ahmed S. Current status of immunotherapy in metastatic colorectal cancer. International Journal of Colorectal Disease. 2019; 34: 13–25.

[3] Kuşoğlu A, Biray Avcı Ç. Cancer stem cells: a brief review of the current status. Gene. 2019; 681: 80–85.

[4] Hai T, Wolford CC, Chang Y. ATF3, a hub of the cellular adaptive-response network, in the pathogenesis of diseases: is modulation of inflammation a unifying component? Gene Expression. 2010; 15: 1–11.

[5] Rohini M, Haritha Menon A, Selvamurugan N. Role of activating transcription factor 3 and its interacting proteins under physiolog-ical and pathological conditions. International Journal of Biological Macromolecules. 2018; 120: 310–317.

[6] Ku HC, Cheng CF. Master Regulator Activating Transcription Factor 3 (ATF3) in Metabolic Homeostasis and Cancer. Frontiers in Endocrinology. 2020; 11: 556.

[7] Yin X, Dewille JW, Hai T. A potential dichotomous role of ATF3, an adaptive-response gene, in cancer development. Oncogene. 2008; 27: 2118–2127.

[8] Li L, Sun RM, Jiang GQ. ATF3 Demethylation Promotes the Transcription of ARL4C, Which Acts as a Tumor Suppressor in Human Breast Cancer. OncoTargets and Therapy. 2020; 13: 3467–3476.

[9] Zu T, Wen J, Xu L, Li H, Mi J, Brakebusch C, et al. Up-Regulation of Activating Transcription Factor 3 in Human Fibroblasts Inhibits Melanoma Cell Growth and Migration Through a Paracrine Pathway. Frontiers in Oncology. 2020; 10: 624.

[10] Wang F, Li J, Wang H, Zhang F, Gao J. Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression. International Journal of Oncology. 2020; 57: 707–720.

[11] Wang CM, Yang WH. Loss of SUMOylation on ATF3 inhibits proliferation of prostate cancer cells by modulating CCND1/2 activity. International Journal of Molecular Sciences. 2013; 14: 8367–8380.

[12] Lin L, Yao Z, Bhuvaneshwar K, Gusev Y, Kallakury B, Yang S, et al. Transcriptional regulation of STAT3 by SPTBN1 and SMAD3 in HCC cAMP-response element-binding proteins ATF3 and CREB2. Carcinogenesis. 2014; 35: 2393–2403.

[13] Wu X, Nguyen BC, Dziunycz P, Chang S, Brooks Y, Lefort K, et al. Opposing roles for calcineurin and ATF3 in squamous skin cancer. Nature. 2010; 465: 368–372.

[14] Bottone FG, Moon Y, Kim JS, Alston-Mills B, Ishibashi M, Eling TE. The anti-invasive activity of cyclooxygenase inhibitors is regulated by the transcription factor ATF3 (activating transcription factor 3). Molecular Cancer Therapeutics. 2005; 4: 693–703.

[15] Edagawa M, Kawauchi J, Hirata M, Goshima H, Inoue M, Okamoto T, et al. Role of activating transcription factor 3 (ATF3) in endoplasmic reticulum (ER) stress-induced sensitization of p53-deficient human colon cancer cells to tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis through up-regulation of death receptor 5 (DR5) by zerumbone and celecoxib. Journal of Biological Chemistry. 2014; 289: 21544–21561.

[16] Taketani K, Kawauchi J, Tanaka-Okamoto M, Ishizaki H, Tanaka Y, Sakai T, et al. Key role of ATF3 in p53-dependent DR5 induction upon DNA damage of human colon cancer cells. Oncogene. 2012; 31: 2210–2221.

[17] Lee SH, Min KW, Zhang X, Baek SJ. 3,3’-diindolylmethane induces activating transcription factor 3 (ATF3) via ATF4 in human colorectal cancer cells. Journal of Nutritional Biochemistry. 2013; 24: 664–671.

[18] Jeong JB, Choi J, Baek SJ, Lee S. Reactive oxygen species mediate tolfenamic acid-induced apoptosis in human colorectal cancer cells. Archives of Biochemistry and Biophysics. 2013; 537: 168–175.

[19] Ishiguro T, Nagawa H. ATF3 gene regulates cell form and migration potential of HT29 colon cancer cells. Oncology Research. 2001; 12: 343–346.

[20] Ishiguro T, Nagawa H, Naito M, Tsuruo T. Inhibitory effect of ATF3 antisense oligonucleotide on ectopic growth of HT29 human colon cancer cells. Japanese Journal of Cancer Research. 2000; 91: 833–836.

[21] Yin X, Wolford CC, Chang YS, McConoughey SJ, Ramsey SA, Aderem A, et al. ATF3, an adaptive-response gene, enhances TGFbeta signaling and cancer-initiating cell features in breast cancer cells. Journal of Cell Science. 2010; 123: 3558–3565.

[22] Bottone FG, Martinez JM, Collins JB, Afshari CA, Eling TE. Gene modulation by the cyclooxygenase inhibitor, sulindac sulfide, in human colorectal carcinoma cells: possible link to apoptosis. Journal of Biological Chemistry. 2003; 278: 25790–25801.

[23] Hackl C, Lang SA, Moser C, Mori A, Fichtner-Feigl S, Hellerbrand C, et al. Activating transcription factor-3 (ATF3) functions as a tumor suppressor in colon cancer and is up-regulated upon heat-shock protein 90 (Hsp90) inhibition. BMC Cancer. 2010; 10: 668.

[24] Song HM, Park GH, Eo HJ, Jeong JB. Naringenin-Mediated ATF3 Expression Contributes to Apoptosis in Human Colon Cancer. Biomolecules & Therapeutics. 2016; 24: 140–146.

[25] Kim KJ, Lee J, Park Y, Lee SH. ATF3 Mediates Anti-Cancer Activity of Trans-10, cis-12-Conjugated Linoleic Acid in Human Colon Cancer Cells. Biomolecules & Therapeutics. 2015; 23: 134–140.

[26] Yan F, Ying L, Li X, Qiao B, Meng Q, Yu L, et al. Overexpression of the transcription factor ATF3 with a regulatory molecular signature associates with the pathogenic development of colorectal cancer. Oncotarget. 2017; 8: 47020–47036.

[27] Okada M, Kawai K, Sonoda H, Shiratori H, Kishikawa J, Nagata H, et al. Epithelial-mesenchymal transition and metastatic ability of CD133+ colorectal cancer stem-like cells under hypoxia. Oncology Letters. 2021; 21: 19–19.

[28] Inoue M, Uchida Y, Edagawa M, Hirata M, Mitamura J, Miyamoto D, et al. The stress response gene ATF3 is a direct target of the Wnt/β-catenin pathway and inhibits the invasion and migration of HCT116 human colorectal cancer cells. PLoS ONE. 2018; 13: e0194160.

[29] Lee SH, Lee J, Herald T, Cox S, Noronha L, Perumal R, et al. Anticancer Activity of a Novel High Phenolic Sorghum Bran in Human Colon Cancer Cells. Oxidative Medicine and Cellular Longevity. 2020; 2020: 2890536.

[30] Park GH, Song HM, Park SB, Son HJ, Um Y, Kim HS, et al. Cytotoxic activity of the twigs of Cinnamomum cassia through the suppression of cell proliferation and the induction of apoptosis in human colorectal cancer cells. BMC Complementary and Alternative Medicine. 2018; 18: 28.

[31] Bottone FG, Moon Y, Kim JS, Alston-Mills B, Ishibashi M, Eling TE. The anti-invasive activity of cyclooxygenase inhibitors is regulated by the transcription factor ATF3 (activating transcription factor 3). Molecular Cancer Therapeutics. 2005; 4: 693–703.

[32] Cao H, Xu E, Liu H, Wan L, Lai M. Epithelial-mesenchymal transition in colorectal cancer metastasis: a system review. Pathology, Research and Practice. 2015; 211: 557–569.

[33] Ishiwata T. Cancer stem cells and epithelial-mesenchymal transition: Novel therapeutic targets for cancer. Pathology International. 2016; 66: 601–608.

Abstracted / indexed in

Science Citation Index Expanded Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,200 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Social Sciences Citation Index Social Sciences Citation Index contains over 3,400 journals across 58 social sciences disciplines, as well as selected items from 3,500 of the world’s leading scientific and technical journals. More than 9.37 million records and 122 million cited references date back from 1900 to present.

Current Contents - Social & Behavioral Sciences Current Contents - Social & Behavioral Sciences provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in the social and behavioral sciences.

Current Contents - Clinical Medicine Current Contents - Clinical Medicine provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in clinical medicine.

SCOPUS Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.

DOAJ DOAJ is a community-curated online directory that indexes and provides access to high quality, open access, peer-reviewed journals.

CrossRef Crossref makes research outputs easy to find, cite, link, assess, and reuse. Crossref committed to open scholarly infrastructure and collaboration, this is now announcing a very deliberate path.

Portico Portico is a community-supported preservation archive that safeguards access to e-journals, e-books, and digital collections. Our unique, trusted process ensures that the content we preserve will remain accessible and usable for researchers, scholars, and students in the future.

Submission Turnaround Time

Conferences

    Top