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Original Research

Open Access

Inhibition of TRAF1 protects renal tubular epithelial cells against hypoxia/reoxygenation injury

  • Wei Yu1
  • Qifeng Mao2,3

1Department of Nephrology, Chongqing General Hospital, 400013 Chongqing, China

2Department of Clinical Laboratory, HwaMei Hospital, University of Chinese Academy of Sciences, 315010 Ningbo, Zhejiang Province, China

3Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, 315010 Ningbo, Zhejiang Province, China

DOI: 10.31083/jomh.2021.060 Vol.17,Issue 3,July 2021 pp.167-173

Submitted: 25 March 2021 Accepted: 15 June 2021

Published: 08 July 2021

*Corresponding Author(s): Qifeng Mao E-mail:

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Background and objective: This study aimed to explore the expression of TRAF1 in vitro kidney injury model, and the function mechanism of TRAF1 in the model growth and apoptosis.

Methods: After transfecting HK2 cells with short hair RNA (shRNA), shTRAF1 gene silencing model was established. The cells were divided into shRNA group and shNC group. For kidney injury model, we used hypoxia/reoxygenation to establish H/R cell lines. MTT assay was used to determine cell viability. PI/FITC staining was used to determine cell apoptosis. The genes expressions were determined by RT-qPCR and western blotting, respectively. The concentration of MDA, SOD, iNOS and LDH was determined by ELISA.

Results: The results of RT-qPCR and western blotting assay revealed that TRAF1 upregulated expression in AKI model cells. The results of MTT assay revealed that shRNA group exhibited significantly higher cell viability and lower cell apoptosis compared with the control group in H/R HK2 cells. In addition, TRAF1 downregulated expression inhibits oxidative stress response in H/R treated HK2 cell. Mechanically, TRAF1 deficiency protects HK2 cell via inhibiting p38-MAPK pathway.

Conclusions: Our study suggests that TRAF1 could be a target in kidney injury treatment.


TRAF1; AKI; Cell apoptosis; H/R injury; p38 MAPK pathway

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Wei Yu,Qifeng Mao. Inhibition of TRAF1 protects renal tubular epithelial cells against hypoxia/reoxygenation injury. Journal of Men's Health. 2021. 17(3);167-173.


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