Effects of acute and chronic high-intensity interval training on serum irisin, BDNF and apelin levels in male soccer referees

This study was aimed to investigate the effects of acute and chronic High Intensity Interval Training (HIIT) on Irisin, BDNF (brain-derived neurotrophic factor), and Apelin levels. The study included twenty-one male soccer referees. Blood from the participants was collected at the beginning of study (1. first measurement: baseline value). HIIT was conducted and blood was immediately collected (2. second measurement: acute effect). Next, HIIT was carried out for 20 minutes of 4 days a week in bouts of running (75 meters in 20 seconds) and walking (25 meters in 20 seconds). Blood was collected at the end of 12 weeks (3. third measurement: chronic effect). HIIT was performed and blood was again collected (4. fourth measurement: acute effect after the chronic effect). There was a gradual increase in irisin, BDNF, and apelin levels (p < 0.001). The increase for irisin was 2% in the second measurement, 106% in the third, and 111% in the fourth compared to the first measurement. The increase for BDNF was 39% in the second measurement, 116% in the third, and 133% in the fourth. Apelin levels were increased by 11%, 19%and 28%, respectively. These results demonstrated that irisin and BDNF might increase only in response to chronic HIIT (4 times a week) while apelin levels might change with both acute and chronic HIIT in healthy trained referees.

HIIT; BDNF; Irisin; Apelin

[1] Hoffmann C, Weigert C. Skeletal muscle as an endocrine organ: the role of myokines in exercise adaptations. Cold Spring Harbor Perspectives in Medicine. 2017; 7: a029793.

[2] Zhang H, Wu X, Liang J, Kirberger M, Chen N. Irisin, an exercise-induced bioactive peptide beneficial for health promotion during aging process. Ageing Research Reviews. 2022; 80: 101680.

[3] Chen KW, Chen L. Epigenetic regulation of BDNF gene during development and diseases. International Journal of Molecular Sciences. 2017; 18: 571.

[4] Murer MG, Yan Q, Raisman-Vozari R. Brain-derived neurotrophic factor in the control human brain, and in Alzheimer’s disease and Parkinson’s disease. Progress in Neurobiology. 2001; 63: 71–124.

[5] Wang J, Dranovsky A, Hen R. The when and where of BDNF and the antidepressant response. Biological Psychiatry. 2008; 63: 640–641.

[6] Leuchtmann AB, Adak V, Dilbaz S, Handschin C. The role of the skeletal muscle secretome in mediating endurance and resistance training adaptations. Frontiers in Physiology. 2021; 12: 709807.

[7] Callahan MJ, Parr EB, Hawley JA, Camera DM. Can high-intensity interval training promote skeletal muscle anabolism? Sports Medicine. 2021; 51: 405–421.

[8] Rosenblat MA, Perrotta AS, Thomas SG. Effect of high-intensity interval training versus sprint interval training on time-trial performance: a systematic review and meta-analysis. Sports Medicine. 2020; 50: 1145–1161.

[9] Gist NH, Freese EC, Cureton KJ. Comparison of responses to two high-intensity intermittent exercise protocols. Journal of Strength and Conditioning Research. 2014; 28: 3033–3040.

[10] Kessler HS, Sisson SB, Short KR. The potential for high-intensity interval training to reduce cardiometabolic disease risk. Sports Medicine. 2012; 42: 489–509.

[11] Jelleyman C, Yates T, O’Donovan G, Gray LJ, King JA, Khunti K, et al. The effects of high-intensity interval training on glucose regulation and insulin resistance: a meta‐analysis. Obesity Reviews. 2015; 16: 942–961.

[12] Batacan RB, Duncan MJ, Dalbo VJ, Tucker PS, Fenning AS. Effects of high-intensity interval training on cardiometabolic health: a systematic review and meta-analysis of intervention studies. British Journal of Sports Medicine. 2017; 51: 494–503.

[13] Castillo D, Cámara J, Lozano D, Berzosa C, Yanci J. The association between physical performance and match-play activities of field and assistants soccer referees. Research in Sports Medicine. 2019; 27: 283–297.

[14] Wadley AJ, Chen Y, Lip GYH, Fisher JP, Aldred S. Low volume-high intensity interval exercise elicits antioxidant and anti-inflammatory effects in humans. Journal of Sports Sciences. 2016; 34: 1–9.

[15] Gibala MJ, Little JP, MacDonald MJ, Hawley JA. Physiological adaptations to low‐volume, high‐intensity interval training in health and disease. The Journal of Physiology. 2012; 590: 1077–1084.

[16] Murawska-Cialowicz E, Wolanski P, Zuwala-Jagiello J, Feito Y, Petr M, Kokstejn J, et al. Effect of HIIT with Tabata protocol on serum irisin, physical performance, and body composition in men. International Journal of Environmental Research and Public Health. 2020; 17: 3589.

[17] Huh JY, Mougios V, Kabasakalis A, Fatouros I, Siopi A, Douroudos II, et al. Exercise-induced irisin secretion is independent of age or fitness level and increased irisin may directly modulate muscle metabolism through AMPK activation. The Journal of Clinical Endocrinology & Metabolism. 2014; 99: E2154–E2161.

[18] Archundia-Herrera C, Macias-Cervantes M, Ruiz-Muñoz B, Vargas-Ortiz K, Kornhauser C, Perez-Vazquez V. Muscle irisin response to aerobic vs HIIT in overweight female adolescents. Diabetology & Metabolic Syndrome. 2017; 9: 101.

[19] Pekkala S, Wiklund PK, Hulmi JJ, Ahtiainen JP, Horttanainen M, Pöllänen E, et al. Are skeletal muscle FNDC5 gene expression and irisin release regulated by exercise and related to health? The Journal of Physiology. 2013; 591: 5393–5400.

[20] Kabak B, Belviranli M, Okudan N. Irisin and myostatin responses to acute high-intensity interval exercise in humans. Hormone Molecular Biology and Clinical Investigation. 2018; 35: 20180008.

[21] He Z, Tian Y, Valenzuela PL, Huang C, Zhao J, Hong P, et al. Myokine response to high-intensity interval vs. resistance exercise: an individual approach. Frontiers in Physiology. 2018; 9: 1735.

[22] Islam MR, Valaris S, Young MF, Haley EB, Luo R, Bond SF, et al. Exercise hormone irisin is a critical regulator of cognitive function. Nature Metabolism. 2021; 3: 1058–1070.

[23] Liu S, Cui F, Ning K, Wang Z, Fu P, Wang D, et al. Role of irisin in physiology and pathology. Frontiers in Endocrinology. 2022; 13: 962968.

[24] Zhang Y, Zhang X, Lin S. Irisin: a bridge between exercise and neurological diseases. Heliyon. 2022; 8: e12352.

[25] Matsumoto J, Takada S, Furihata T, Nambu H, Kakutani N, Maekawa S, et al. Brain-derived neurotrophic factor improves impaired fatty acid oxidation via the activation of adenosine monophosphate-activated protein kinase-ɑ—proliferator-activated receptor-r coactivator-1ɑ signaling in skeletal muscle of mice with heart failure. Circulation: Heart Failure. 2021; 14: e005890.

[26] Tsai C, Pan C, Tseng Y, Chen F, Chang Y, Wang T. Acute effects of high-intensity interval training and moderate-intensity continuous exercise on BDNF and irisin levels and neurocognitive performance in late middle-aged and older adults. Behavioural Brain Research. 2021; 413: 113472.

[27] Cabral-Santos C, Castrillón CI, Miranda RA, Monteiro PA, Inoue DS, Campos EZ, et al. Inflam-matory cytokines and BDNF response to high-intensity intermittent exercise: effect the exercise volume. Frontiers in Physiology. 2016; 7: 509.

[28] Saucedo Marquez CM, Vanaudenaerde B, Troosters T, Wenderoth N. High-intensity interval training evokes larger serum BDNF levels compared with intense continuous exercise. Journal of Applied Physiology. 2015; 119: 1363–1373.

[29] Li Q, Zhang L, Zhang Z, Wang Y, Zuo C, Bo S. A shorter-bout of HIIT is more effective to promote serum BDNF and VEGF-A levels and improve cognitive function in healthy young men. Frontiers in Physiology. 2022; 13: 898603.

[30] Bastioli G, Arnold JC, Mancini M, Mar AC, Gamallo-Lana B, Saadipour K, et al. Voluntary exercise boosts striatal dopamine release: evidence for the necessary and sufficient role of BDNF. The Journal of Neuroscience. 2022; 42: 4725–4736.

[31] Chan Y, Jang J, Ho C. Effects of physical exercise on children with attention deficit hyperactivity disorder. Biomedical Journal. 2022; 45: 265–270.

[32] Tatemoto K, Hosoya M, Habata Y, Fujii R, Kakegawa T, Zou M, et al. Isolation and characterization of a novel endogenous peptide ligand for the human APJ receptor. Biochemical and Biophysical Research Communications. 1998; 251: 471–476.

[33] Luo J, Liu W, Feng F, Chen L. Apelin/APJ system: a novel therapeutic target for locomotor system diseases. European Journal of Pharmacology. 2021; 906: 174286.

[34] Bilski J, Jaworek J, Pokorski J, Nitecki J, Nitecka E, Pokorska J, et al. Effects of time of day and the wingate test on appetite perceptions, food intake and plasma levels of adipokines. Journal of Physiology and Pharmacology. 2016; 67: 667–676.

[35] Kon M, Ebi Y, Nakagaki K. Effects of acute sprint interval exercise on follistatin-like 1 and apelin secretions. Archives of Physiology and Biochemistry. 2021; 127: 223–227.

[36] Sabouri M, Norouzi J, Zarei Y, Sangani MH, Hooshmand Moghadam B. Comparing high-intensity interval training (HIIT) and continuous training on apelin, APJ, no, and cardiotrophin-1 in cardiac tissue of diabetic rats. Journal of Diabetes Research. 2020; 2020: 1–7.

[37] Krist J, Wieder K, Klöting N, Oberbach A, Kralisch S, Wiesner T, et al. Effects of weight loss and exercise on apelin serum concentrations and adipose tissue expression in human obesity. Obesity Facts. 2013; 6: 57–69.

[38] Sheibani S, Hanachi P, Refahiat MA. Effect of aerobic exercise on serum concentration of apelin, TNFα and insulin in obese women. Iranian Journal of Basic Medical Sciences. 2012; 15: 1196–1201.

[39] Kadoglou NPE, Vrabas IS, Kapelouzou A, Angelopoulou N. The association of physical activity with novel adipokines in patients with type 2 diabetes. European Journal of Internal Medicine. 2012; 23: 137–142.

[40] Xie H, Tang S, Cui R, Huang J, Ren X, Yuan L, et al. Apelin and its receptor are expressed in human osteoblasts. Regulatory Peptides. 2006; 134: 118–125.

[41] Higuchi K, Masaki T, Gotoh K, Chiba S, Katsuragi I, Tanaka K, et al. Apelin, an APJ receptor ligand, regulates body adiposity and favors the messenger ribonucleic acid expression of uncoupling proteins in mice. Endocrinology. 2007; 148: 2690–2697.