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Original Research

Open Access


  • Saad Alshahrani1
  • Gulfam Ahmad2
  • Haroon Latif Khan3
  • Ahmet Ayaz4
  • Ali Hassan A. Ali1,5

1Department of Surgery, College of Medicine, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia

2Discipline of Pathology, School of Medical Sciences, Sydney Medical School, University of Sydney, Sydney, Australia

3Lahore Institute of Fertility and Endocrinology (LIFE), Lahore, Pakistan

4Department of Environmental Health Sciences, University of Massachusetts, Amherst, Massachusetts, USA

5Faculty of Medicine, Department of Anatomy, Al-Azhar University, Cairo, Egypt

DOI: 10.22374/jomh.v15i2.144 Vol.15,Issue 2,April 2019 pp.58-65

Published: 01 April 2019

*Corresponding Author(s): Saad Alshahrani E-mail:

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Background and objective

The aim of the present study was to identify the impact of defective standard sperm parameters indi-vidually and in combination on DNA damage in a large cohort of infertile men.

Material and methods

Retrospective analysis of semen characteristics was conducted on 436 patients. DNA fragmentation analysis was performed by using the terminal deoxynucleotidyl transferase (TdT)-mediated fluoresce-in-dUTP nick end labeling (TUNEL) assay. Sperm parameters were arranged into different categories such as normospermia asthenospermic, teratospermic, asthenoteratospermic, and oligoasthenoter-atospermic. GraphPad Prism version 7 software was used for data analysis.


Our results suggest that the mean percentage of DNA damage was proportionally higher than the semen abnormalities. Sperm with 3 abnormal parameters showed significantly higher DNA damage, suggesting that sperm having more than 2 abnormalities are more likely to have higher DNA damage.


Sperm motility had significant correlation and is supposed to be a predictor for these tests, while mor-phology was the second standard sperm parameter inversely correlated with sperm DNA damage. Patients demonstrating low levels of leukocytospermia should be advised sperm DNA testing before assisted reproductive technology treatment. However, there is a clear need for more research studies to further address these issues.


semen analysis, DNA fragmentation, male infertility, sperm abnormalities

Cite and Share

Saad Alshahrani,Gulfam Ahmad,Haroon Latif Khan,Ahmet Ayaz,Ali Hassan A. Ali. IMPACT OF SINGLE AND MULTIPLE SPERM ABNORMALITIES AND LOW-LEVEL LEUKOCYTOSPERMIA ON SPERM DNA. Journal of Men's Health. 2019. 15(2);58-65.


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