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Original Research

Open Access

The Differential Impact Of High-Intensity Swimming Exercise and Inflammatory Bowel Disease On IL-1b, TNF-a, and COX-2 Gene Expression in the Small Intestine and Colon in Mice

  • Eun-Ju Choi1
  • Wi-Young So2

1Assistant Professor, Department of Physical Education, Daegu Catholic University, Gyeongsan, Korea

2Associate Professor, Sports and Health Care Major, College of Humanities and Arts, Korea National University of Transportation, Chungju-si, Korea

DOI: 10.22374/1875-6859.14.2.4 Vol.14,Issue 2,February 2018 pp.22-29

Published: 22 February 2018

*Corresponding Author(s): Wi-Young So E-mail:

PDF (1,013.2 kB)


Background and Objective

We aimed to examine the impact of high-intensity swimming exercise and inflammatory bowel disease (IBD) on IL-1β, TNF-α, and COX-2 gene expression in the small intestine and colon of mice.

Material and Methods

Forty male C57BL/6 mice were divided into 4 groups: the control group (CON), swimming exercise group (EX), 50% ethanol (EtoH) control group (50%EtoH CON), and 2,4,6-trinitrobenzene sulfonic acid group (TNBS). The EX group performed 4 weeks of exercise. Intrarectal TNBS injection induced IBD in the TNBS group; the 50%EtoH CON group received control injections. Reverse transcription and real-time polymerase chain reaction were used to examine IL-1β, TNF-α, and COX-2 mRNA expression in the small intestine and colon.


IL-1β, TNF-α, and COX-2 mRNA expression was significantly increased in the EX group compared to that in the CON group (p’s<0.05). IL-1β and COX-2 mRNA expression was significantly increased in the TNBS group compared to that in the 50%EtoH CON group (p’s<0.05).


Thus, inflammatory cytokine IL-1β and COX-2 expression in the small intestine and colon was increased in both high-intensity swimming exercise and IBD models. However, TNF-α was increased only in the swimming exercise model. Further research is required to confirm these observations and establish swim-ming exercise regimes appropriate for patients with IBD.

Cite and Share

Eun-Ju Choi,Wi-Young So. The Differential Impact Of High-Intensity Swimming Exercise and Inflammatory Bowel Disease On IL-1b, TNF-a, and COX-2 Gene Expression in the Small Intestine and Colon in Mice. Journal of Men's Health. 2018. 14(2);22-29.


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