Article Data

  • Views 524
  • Dowloads 121

Original Research

Open Access

Effects of Resistance Exercise Intensity on Cytokine and Chemokine Gene Expression in Atopic Dermatitis Mouse Model

  • Eun-Ju Choi1
  • Wi-Young So2

1Assistant Professor, Department of Physical Education, Daegu Catholic University, Gyeongsan, Korea

2Associate Professor, Sports and Health Care Major, College of Humanities and Arts, Korea National University of Transportation, Chungju-si, Korea

DOI: 10.22374/1875-6859.14.2.3 Vol.14,Issue 2,February 2018 pp.14-21

Published: 22 February 2018

*Corresponding Author(s): Wi-Young So E-mail:

PDF (1.27 MB)


Background and Objective

Although the evidence is unclear, literature indicates that resistance exercise reduces inflammation in colorectal disease. The purpose of this study was to identify the effects of colon tissue on cytokine and chemokine gene expression with changes in resistance exercise intensity.

Material and Methods

We divided male BABL/c mice into 6 groups (each group n=10, total=60) (control group: CON, low re-sistance exercise group: EX_L, high resistance exercise group: EX_H, atopic dermatitis group: AD, atopic dermatitis+low resistance exercise group: AD+EX_L, atopic dermatitis+high resistance exercise group: AD+EX_H) and subjected them to ladder climbing resistance exercise for 4 weeks. After 24 h of each exercise schedule, a real-time polymerase chain reaction was performed to determine mRNA expression of interleukin-6 (IL-6) and chemokine ligand 20 (CCL20).


The AD group showed significantly higher mRNA expression of IL-6 and CCL20 compared with the CON, EX_L, EX_H, AD+EX_L, and AD+EX_H groups (p<0.05).


In conclusion, both high and low resistance exercise effectively decreases the concentration of IL-6 and CCL20 in mice with and without AD.

Cite and Share

Eun-Ju Choi,Wi-Young So. Effects of Resistance Exercise Intensity on Cytokine and Chemokine Gene Expression in Atopic Dermatitis Mouse Model. Journal of Men's Health. 2018. 14(2);14-21.


1. Choi EJ, Debnath T, Tang Y, et al. Topical application of Moringa oleifera leaf extract ameliorates experimentally induced atopic dermatitis by the regulation of Th1/Th2/Th17 balance. Biomed Pharmacother 2016;84:870–7.

2. Castan L, Magnan A, Bouchaud G. Chemokine recep-tors in allergic diseases. Allergy 2017;72(5):682–90.

3. Hiramoto K, Kobayashi H, Sekiyama AF, et al. Mild exercise suppresses exacerbation of dermatitis by increasing cleavage of the b-endrophin from proopi-omelanocortin in NC/Nga mice. J Clin Biochem Nutr 2013;52(1):58–63.

4. Strom MA, Silverberg JI. Associations of physical activity and sedentary behavior with atopic disease in united states children. J Pediatr 2016;174:247–53.

5. Willett WC, Stampfer MJ, Colditz GA, et al. Relation of meat, fat and fiber intake to the risk of colon cancer in a prospective study among women. N Engl J Med 1992;323(24):1664–72.

6. Sanchez NF, Stierman B, Saab S, et al. Physical activity reduces risk for colon polyps in a multiethnic colorectal cancer screening population. BMC Research Notes 2012;5:312.

7. Zaslau S, Jansen R, Riggs DR, et al. Possible preven-tion and treatment of prostate cancer by exercise. West Virginia Med J 2012;108(3):42–7.

8. Courneya KS, Vardy JL, O’callaghan CJ, et al. Effects of a structured exercise program on physical activity and fitness in colon cancer survivors: one year feasibility results from the CHALLENGE Trial. Cancer Epidemiol Biomark Prevent 2016;25(6):969–77.

9. Martin D. Physical activity benefits and risks on the gas-trointestinal system. Southern Med J 2011;104(12):831–7.

10. Mardare C, Kruger K, Liebisch G, et al. Endurance and resistance training affect high fat diet-induced increase of ceramides, inflammasome expression, and systemic inflammation in mice. J Diabet Res 2016;2016:4536470.

11. Lee HS, Choi EJ, Lee KS, et al. Oral administration of

p- Hydroxycinnamic Acid attenuates atopic dermatitis by downregulating Th1 and Th2 cytokine production and keratinocyte activation. PLOS ONE 2016;11(2):e0150952.

12. Tsang MS, Jiao D, Chan BC, et al. Anti-inflammatory activities of pentaherbs formula, berberine, gallic acid chlorogenic acid in atopic dermatitis-like skin inflam-mation. Molecules 2016;21(4):e519.

13. Choi EJ, Iwasa M, Han KI, et al. Heat-killed Enterococ-cus faecalis EF-2001 ameliorates atopic dermatitis in a murine model. Nutrients 2016;8(3):636–45.

14. Bochkov V, Kadl A, Huber J, et al. Protective role of phospholipid oxidation products in endotoxin-induced tissue damage. Nature 2002;419(6902):77–81.

15. Frick VO, Rubic C, Keilholz U, et al. Chemokine/chemokine receptor pair CCL20/CCR6 in human colo-rectal malignancy: An overview. World J Gastroenterol 2016;22(2):833–41.

16. Choi SC, Choi EJ, Oh HM, et al. DA-9601, a standardized extract of Artemisia asiatica, blocks TNF-alpha-induced IL-8 and CCL20 production by inhibiting p38 kinase and NF-kappaB pathways in human gastric epithelial cells. World J Gastroenterol 2006;12(30):4850–8.

17. Shoji J, Nakanishi Y, Ishimori A, et al. Involvement of chemokines and a CD4-positive T cell subset in the development of conjunctival secondary lymphoid fol-licles in an atopic keratoconjunctivitis mouse model. Internat Arch Allerg Immunol 2015;167(3):147–57.

18. Kraemer WJ, Flanagan SD, Volek JS, et al. Resistance exercise induces rigion-specific adaptations in anterior pituitary gland structure and function in rats. J Appl Physiol 2013;115(11):1641–7.

19. Kim SH, Kim EK, Choi EJ. High-intensity swimming exercise increases dust mite extract and 1-chloro-2,4-dinitrobenzene-derived atopic dermatitis in BALB/c mice. Inflammation 2014;37(4):1179–85.

20. Gidlund EK, Von Walend F, Venojarvi M, et al. Human in skeletal muscle protein levels increase after resistance training in men with impaired glucose metabolism. Physiolog Rep 2016;4(23):13063.

21. Gjevestad GO, Holven KB, Ulven SM. Effects of exercise on gene expression of inflammatory markers in human peripheral blood cells: A systematic review. Curr Cardiovasc Risk Rep 2015;9(7):34.

22. Martin R, Miguel S, Chain F, et al. Faecalibacterium prausnitzii prevents physiological damages in a chronic low-grade inflammation murine model. BMC Microbiol 2015;15:67.

23. Cullen T, Thomas AW, Webb R, et al. Interleukin-6 and associated cytokine responses to an acute bout of high-intensity interval exercise: the effect of exercise intensity and volume. Appl Physiol Nutrit Metab 2016;41(8):803–8.

Abstracted / indexed in

Science Citation Index Expanded Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,200 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Social Sciences Citation Index Social Sciences Citation Index contains over 3,400 journals across 58 social sciences disciplines, as well as selected items from 3,500 of the world’s leading scientific and technical journals. More than 9.37 million records and 122 million cited references date back from 1900 to present.

Current Contents - Social & Behavioral Sciences Current Contents - Social & Behavioral Sciences provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in the social and behavioral sciences.

Current Contents - Clinical Medicine Current Contents - Clinical Medicine provides easy access to complete tables of contents, abstracts, bibliographic information and all other significant items in recently published issues from over 1,000 leading journals in clinical medicine.

SCOPUS Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.

DOAJ DOAJ is a community-curated online directory that indexes and provides access to high quality, open access, peer-reviewed journals.

CrossRef Crossref makes research outputs easy to find, cite, link, assess, and reuse. Crossref committed to open scholarly infrastructure and collaboration, this is now announcing a very deliberate path.

Portico Portico is a community-supported preservation archive that safeguards access to e-journals, e-books, and digital collections. Our unique, trusted process ensures that the content we preserve will remain accessible and usable for researchers, scholars, and students in the future.

Submission Turnaround Time