EFFECTS OF A SINGLE BOUT OF AEROBIC EXERCISE ON SKELETAL MUSCLE PROTEIN TURNOVER IN MICE
1Research Professor, Department of Toxicology, College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea
2Associate Professor, Sports and Health Care Major, College of Humanities and Arts, Korea National University of Transportation, Chungju-si, Republic of Korea
DOI: 10.22374/1875-6818.104.22.168 Vol.14,Issue 1,January 2018 pp.6-15
Published: 01 January 2018
Background and Objective
Aerobic exercise has a strong effect on skeletal muscle metabolism, in both males and females of all ages. However, the effect of a single bout of aerobic exercise on the regulation of protein balance remains unknown. In the present study, we investigated the effect of a single bout of treadmill-based exercise on the levels of various protein synthesis-related eukaryotic proteins (initiation factor 2a, eukaryotic initiation factor 4E, eukaryotic initiation factor 4E-binding protein 1, and eukaryotic elongation factor 2), breakdown-related proteins (microtubule-associated protein 1 light chain 3 alpha (LC3), autophagy-related 7, and muscle RING-finger protein 1 (MuRF1)), and polyubiquitination in 3-month-old male ICR mice.
Material and Methods
Twenty-four male mice were randomized into four time-point groups; each group of mice was run on a rodent treadmill for 10 min at 10 m/min at a slope of 5° between 7:00 p.m. and 8:00 p.m. for 2 days during the adaptation period. On the third day, exercise was performed for 50 min at a speed of 12.3 m/min; the control mice did not perform any exercise. Gastrocnemius muscles were collected immediately after the mice were sacrificed by cervical dislocation at 0, 3, 6, or 12 hours post-exercise.
Levels of synthesis-related proteins were significantly reduced at 3 and 6 hours into the recovery period,
whereas levels of breakdown-related proteins, including that of the autophagy marker LC3, increased im-mediately after exercise but not during the recovery period. MuRF1 level was determined in the gastrocnemius muscle to identify the factors involved in this increase. We found that increased MuRF1 levels were associated with an increase in polyubiquitination during the recovery period.
Our results suggest a potential role of optimal time points in muscle protein metabolism during recovery from a single bout of treadmill exercise.
Yong-An Kim,Wi-Young So. EFFECTS OF A SINGLE BOUT OF AEROBIC EXERCISE ON SKELETAL MUSCLE PROTEIN TURNOVER IN MICE. Journal of Men's Health. 2018. 14(1);6-15.
1. Rasmussen BB, Richter EA. The balancing act between the cellular processes of protein synthesis and break-down: exercise as a model to understand the molecular mechanisms regulating muscle mass. J Appl Physiol 2009;106(4):1365–66.
2. Miyazaki M, Esser KA. Cellular mechanisms regulating protein synthesis and skeletal muscle hypertrophy in animals. J Appl Physiol 2009;106(4):1367–73.
3. Wauson EM,, Zaganjor E, Cobb MH. Amino acid regulation of autophagy through the GPCR TAS1R1-TAS1R3. Autophagy 2013;9(3):418–9.
4. Kim KH, Jeong YT, Oh H, et al. Autophagy deficiency leads to protection from obesity and insulin resistance by inducing Fgf21 as a mitokine. Nat Med 2013;19(1):83–92.
5. Rose AJ, Richter EA. Regulatory mechanisms of skeletal muscle protein turnover during exercise. J Appl Physiol 2009;106(5):1702–11.
6. Pikosky MA, Gaine PC, Martin WF, et al. Aerobic exer-cise training increases skeletal muscle protein turnover in healthy adults at rest. J Nutr 2006;136(2):379–83.
7. Louis E, Raue U, Yang Y, Jemiolo B, Trappe S. Time course of proteolytic, cytokine, and myostatin gene ex-pression after acute exercise in human skeletal muscle. J Appl Physiol 2007;103(5):1744–51.
8. Nyfeler B, Bergman P, Triantafellow E, et al. Relieving autophagy and 4EBP1 from rapamycin resistance. Mol Cell Biol 2011;31(14):2867–76.
9. Yang Z, Klionsky DJ. Mammalian autophagy: core molecular machinery and signaling regulation. Curr Opin Cell Biol 2010;22(2):124–31.
10. Haas AL, Baboshina O, Williams B, Schwartz LM. Coordinated induction of the ubiquitin conjugation pathway accompanies the developmentally pro-grammed death of insect skeletal muscle. J Biol Chem 1995;270(16):9407–12.
11. Schwartz LM, Myer A, Kosz L, Engelstein M, Maier
C. Activation of polyubiquitin gene expression dur-ing developmentally programmed cell death. Neuron 1990;5(4):411–9.
12. Lauzon RJ, Patton CW, Weissman IL. A morphological and immunohistochemical study of programmed cell death in Botryllus schlosseri (Tunicata, Ascidiacea). Cell Tissue Res 1993;272(1):115–27.
13. Holcik M, Sonenberg N. Translational control in stress and apoptosis. Nat Rev Mol Cell Biol 2005;6(4):318–27.
14. Rose AJ, Broholm C, Kiillerich K, et al. Exercise rapidly increases eukaryotic elongation factor 2 phosphoryla-tion in skeletal muscle of men. J Physiol 2005;569(Pt 1):223–8.
15. Rose AJ, Bisiani B, Vistisen B, Kiens B, Richter EA. Skeletal muscle eEF2 and 4EBP1 phosphorylation dur-ing endurance exercise is dependent on intensity and muscle fiber type. Am J Physiol Regul Integr Comp Physiol 2009;296(2):R326–33.
16. Rennie MJ, Tipton KD. Protein and amino acid me-tabolism during and after exercise and the effects of nutrition. Annu Rev Nutr. 2000; 20:457–83.
17. Williamson DL, Bolster DR, Kimball SR, Jefferson LS. Time course changes in signaling pathways and protein synthesis in C2C12 myotubes following AMPK activation by AICAR. Am J Physiol Endocrinol Metab 2006;291(1):E80–9.
18. Williamson DL, Kubica N, Kimball SR, Jefferson LS. Exercise-induced alterations in extracellular signal-regulated kinase 1/2 and mammalian target of rapamycin (mTOR) signalling to regulatory mechanisms of mRNA translation in mouse muscle. J Physiol 2006;573(Pt 2):497–510.
19. Proud CG. Signalling to translation: how signal transduc-tion pathways control the protein synthetic machinery. Biochem J 2007;403(2):217–34.
20. Fry CS, Drummond MJ, Glynn EL, et al. Skeletal muscle autophagy and protein breakdown following resist-ance exercise are similar in younger and older adults. J Gerontol A Biol Sci Med Sci 2013;68(5):599–607.
21. Gautsch TA, Anthony JC, Kimball SR, Paul GL, Lay-man DK, Jefferson LS. Availability of eIF4E regulates skeletal muscle protein synthesis during recovery from exercise. Am J Physiol 1998;274(2 Pt 1):C406–14.
22. Atherton PJ, Babraj J, Smith K, Singh J, Rennie MJ, Wackerhage H. Selective activation of AMPK-PGC-1alpha or PKB-TSC2-mTOR signaling can explain specific adaptive responses to endurance or resistance training-like electrical muscle stimulation. FASEB J 2005;19(7):786–88.
23. Phillips SM, Tipton KD, Aarsland A, Wolf SE, Wolfe RR. Mixed muscle protein synthesis and breakdown after resistance exercise in humans. Am J Physiol 1997;273(1 Pt 1):E99–107.
24. Kim YA, Kim YS, Song W. Autophagic response to a single bout of moderate exercise in murine skeletal muscle. J Physiol Biochem 2012;68(2):229–35.
25. Masiero E, Agatea L, Mammucari C, et al. Autophagy is required to maintain muscle mass. Cell Metab 2009;10(6):507–15.
26. Bylund-Fellenius AC, Ojamaa KM, Flaim KE, et al. Protein synthesis versus energy state in contracting mus-cles of perfused rat hindlimb. Am J Physiol 1984;246(4 Pt 1):E297–305.
27. Jefferson LS, Li JB, Rannels SR. Regulation by insulin of amino acid release and protein turnover in the perfused rat hemicorpus. J Biol Chem 1977;252(4):1476–83.
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