THE EFFECT OF OCCASIONAL ALCOHOL DRINKING ON SEMEN QUALITY AND SPERM MORPHOLOGY AMONG YOUNG AND HEALTHY POLISH MEN

Background

Ethanol (EtOH) is an agent that seems to exert an especially harmful effect on male fertility. The impact of high EtOH intake on fertility was demonstrated in numerous researches, with data suggesting that this effect may have been due to decreased semen quality; however, similar negative effects were not identified among occasional EtOH drinkers. There are currently no recommendations for alcohol consumption for men who plan to have a child other than avoiding high EtOH intake. Thus, studies on the effect of moder-ate and occasional EtOH drinking on semen quality are needed to develop appropriate recommendations for men planning to have a child in the future. The aim of this study was to determine whether changes in semen-quality parameters and sperm morphology occur in healthy young men who occasionally exceed the WHO-recommended weekly dose of EtOH but are not alcohol dependent and do not frequently consume high amounts of EtOH.

Methods

The study sample consisted of 172 young men residing in urban areas. The semen quality and morphology of men who consumed more than 140 g of ethanol (high-risk group, HR, n = 44) weekly was compared with that of low-risk group members (LR, n = 128) who reported lower alcohol consumption.

Results

The only between-group difference in semen characteristics was the identification of a higher percentage of macrocephalic sperm in the HR group (P = 0.011). Alcohol intake was the sole factor influencing the percentage of macrocephalic sperm (b = 0.171, P = 0.025, multiple linear regression).

Conclusions

We concluded that occasional alcohol consumption did not alter fertility but caused the accumulation of macrocephalic sperm potentially containing damaged DNA. Therefore, we recommend that men who plan to father children stop drinking alcohol at least 3 months before engaging in sexual intercourse that may lead to pregnancy.

alcohol intake; semen quality; sperm morphology; macrocephalic sperm

1. Nachtigall RD. International disparities in access to infertility services. Fertil Steril 2006;85(4):871–75.

2. Brugo S, Olmedo C, Chillik S, et al. Sperm morphol-ogy, motility, and concentration in fertile and infertile men. N Eng J Med 2001;345(19):1388–93.

3. Barbonetti A, Castellini C, Di Giammarco N, et al. In vitro exposure of human spermatozoa to bisphenol A induces pro-oxidative/apoptotic mitochondrial dysfunc-tion. Reprod Toxicol 2016;66:61–67.

4. Bergamo P, Volpe MG, Lorenzetti S, et al. Human se-men as an early, sensitive biomarker of highly polluted living environment in healthy men: A pilot biomonitor-ing study on trace elements in blood and semen and their relationship with sperm quality and RedOx status. Reprod Toxicol 2016;66:1–9.

5. Yang H, Chen Q, Zhou N, et al. Lifestyles associated with human semen quality: results from MARHCS cohort study in Chongqing, China. Medicine 2015;94(28):e1166.

6. Condorelli RA, Calogero AE, Vicari E, La Vignera S. Chronic consumption of alcohol and sperm parameters: our experience and the main evidences. Andrologia 2015;47(4):368–79.

7. Hansen ML, Thulstrup AM, Bonde JP, et al. Does last week’s alcohol intake affect semen quality or reproduc-tive hormones? A cross-sectional study among healthy young Danish men. Reprod Toxicol 2012;34:457–62.

8. Jensen TK, Swan S, Jørgensen N, et al. Alcohol and male reproductive health: a cross-sectional study of 8344 healthy men from Europe and the USA. Hum Reprod 2014;29(8):1801–809.

9. Ricci E, Al Beitawi S, Cipriani S, et al. Semen quality and alcohol intake: a systematic review and meta-analysis. Reprod Biomed Online 2017;34:38–47.

10. National Institute on Alcohol Abuse and Alcoholism, Assessing alcohol problems: a guide for clinicians and researchers. 2003. Available at: http://pubs.niaaa. nih.gov/publications/Assesing%20Alcohol/index.htm

11. A. Kalinowski, K. Humphreys. Governmental standard drink definitions and low-risk alcohol consumption guidelines in 37 countries. Addiction 2016;111:1293–98.

12. Babor TF, Higgins-Biddle JC. Brief intervention: for hazardous and harmful drinking: Geneva: World Health Organization; 2001. Available at: http://whqlibdoc.who. int/hq/2001/WHO_MSD_MSB_01.6b.pdf?ua=1%20.

13. World Health Organization. WHO Laboratory, Manual for the Examination and Processing of Human Semen, fifth ed., WHO Press, Geneva, 2010.

14. Jóźków P, Mędraś M, Lwow F, at al. Associations between physical activity and semen quality in young healthy men. Fertil Steril 2017;107(2):373–78.

15. Cooper TG, Noonan E, von Eckardstein S, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update 2010;16(3):231–45.

16. Auger J, Eustache F, Andersen AG, et al. Sperm mor-phological defects related to environment, lifestyle and medical history of 1001 male partners of preg-nant women from four European cities. Hum Reprod 2001;16(12):2710–17.

17. Menkveld R. Clinical significance of the low normal sperm morphology value as proposed in the fifth edi-tion of the WHO Laboratory Manual for the examina-tion and processing of human semen. Asian J Androl 2010;12:47–58.

18. Menkveld R, Holleboom CAG, Rhemrev JPT. Measure-ment and significance of sperm Morphology. Asian J Androl 2011;13:59–68.

19. Muthusami KR, Chinnaswamy P. Effect of chronic alcoholism on male fertility hormones and semen qual-ity. Fertil Steril 2005;84(4):919–24.

20. Koop DR. Alcohol metabolism’s damaging effects on the cell: a focus on reactive oxygen generation by the enzyme cytochrome P450 2E1. Alcohol Res Health 2006;29(4):274–80.

21. Wu D, Cederbaum AJ. Alcohol, oxidative stress, and free radical damage. Alcohol Res & Health 2003;27(4):277–84.

22. Aitken RJ, Smith TB, Jobling MS, et al. Oxidative stress and male reproductive health. Asian J Androl 2014;16:31–38.

23. Jena NR. DNA damage by reactive species: mechanisms, mutation and repair. J Biosci 2012;37:503–17.

24. Talebi AR, Sarcheshmeh AA, Khalili MA, Tabibnejad N. Effects of ethanol consumption on chromatin condensa-tion and DNA integrity of epididymal spermatozoa in rat. Alcohol 2011;45:403–409.

25. Guthauser BM, Albert M, Ferfouri F, et al. Inverse cor-relation between chromatin condensation and sperm head size in a case of enlarged sperm heads. Reprod Biomed Online 2011;23:711–16.

26. Iommiello VM, Albani E, Di Rosa A, et al. Ejaculate oxidative stress is related with sperm DNA fragmenta-tion and round cells. Int J Endocrinol 2015;2015:article ID 321901.

27. Guthauser B, Pollet-Villard X, Boitrelle F, Vialard F. Is intracouple assisted reproductive technology an option for men with large-headed spermatozoa? A literature review and a decision guide proposal. Basic Clin An-drol 2016;26:8.

28. Wiweko B, Utami P. Predictive value of sperm deoxy-ribonucleic acid (DNA) fragmentation index in male infertility, Basic Clin Androl 2017;27:1.

29. Simon L, Zini A, Dyachenko A, et al. A systematic review and meta-analysis to determine the effect of sperm DNA damage on in vitro fertilization and intra-cytoplasmic sperm injection outcome. Asian J Androl 2017;19: 80–90.

30. Pflaum T, Hausler T, Baumung C, et al. Carcinogenic compounds in alcoholic beverages: an update. Arch Toxicol 2016;90:23493–67.

31. Amann RP. The cycle of the seminiferous epithelium in humans: a need to revisit? J Androl 2008;29:469–87. doi: 10.2164/jandrol.107.004655.