Effects of Resistance Exercise Intensity on Cytokine and Chemokine Gene Expression in Atopic Dermatitis Mouse Model

Main Article Content

Eun-Ju Choi
Wi-Young So


Atopic Dermatitis, Exercise


Background and Objective

Although the evidence is unclear, literature indicates that resistance exercise reduces inflammation in colorectal disease. The purpose of this study was to identify the effects of colon tissue on cytokine and chemokine gene expression with changes in resistance exercise intensity.

Material and Methods

We divided male BABL/c mice into 6 groups (each group n=10, total=60) (control group: CON, low resistance exercise group: EX_L, high resistance exercise group: EX_H, atopic dermatitis group: AD, atopic dermatitis+low resistance exercise group: AD+EX_L, atopic dermatitis+high resistance exercise group: AD+EX_H) and subjected them to ladder climbing resistance exercise for 4 weeks. After 24 h of each exercise schedule, a real-time polymerase chain reaction was performed to determine mRNA expression of interleukin-6 (IL-6) and chemokine ligand 20 (CCL20).


The AD group showed significantly higher mRNA expression of IL-6 and CCL20 compared with the CON, EX_L, EX_H, AD+EX_L, and AD+EX_H groups (p<0.05).


In conclusion, both high and low resistance exercise effectively decreases the concentration of IL-6 and CCL20 in mice with and without AD.


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1. Choi EJ, Debnath T, Tang Y, et al. Topical application of Moringa oleifera leaf extract ameliorates experimentally induced atopic dermatitis by the regulation of Th1/Th2/ Th17 balance. Biomed Pharmacother 2016;84:870–7.
2. Castan L, Magnan A, Bouchaud G. Chemokine receptors in allergic diseases. Allergy 2017;72(5):682–90.
3. Hiramoto K, Kobayashi H, Sekiyama AF, et al. Mild exercise suppresses exacerbation of dermatitis by increasing cleavage of the b-endrophin from proopiomelanocortin in NC/Nga mice. J Clin Biochem Nutr 2013;52(1):58–63.
4. Strom MA, Silverberg JI. Associations of physical activity and sedentary behavior with atopic disease in united states children. J Pediatr 2016;174:247–53.
5. Willett WC, Stampfer MJ, Colditz GA, et al. Relation of meat, fat and fiber intake to the risk of colon cancer in a prospective study among women. N Engl J Med 1992;323(24):1664–72.
6. Sanchez NF, Stierman B, Saab S, et al. Physical activity reduces risk for colon polyps in a multiethnic colorectal cancer screening population. BMC Research Notes 2012;5:312.
7. Zaslau S, Jansen R, Riggs DR, et al. Possible prevention and treatment of prostate cancer by exercise. West Virginia Med J 2012;108(3):42–7.
8. Courneya KS, Vardy JL, O’callaghan CJ, et al. Effects of a structured exercise program on physical activity and fitness in colon cancer survivors: one year feasibility results from the CHALLENGE Trial. Cancer Epidemiol Biomark Prevent 2016;25(6):969–77.
9. Martin D. Physical activity benefits and risks on the gastrointestinal system. Southern Med J 2011;104(12):831–7.
10. Mardare C, Kruger K, Liebisch G, et al. Endurance and resistance training affect high fat diet-induced increase of ceramides, inflammasome expression, and systemic inflammation in mice. J Diabet Res 2016;2016:4536470.
11. Lee HS, Choi EJ, Lee KS, et al. Oral administration of p-Hydroxycinnamic Acid attenuates atopic dermatitis by downregulating Th1 and Th2 cytokine production and keratinocyte activation. PLOS ONE 2016;11(2):e0150952.
12. Tsang MS, Jiao D, Chan BC, et al. Anti-inflammatory activities of pentaherbs formula, berberine, gallic acid chlorogenic acid in atopic dermatitis-like skin inflammation. Molecules 2016;21(4):e519.
13. Choi EJ, Iwasa M, Han KI, et al. Heat-killed Enterococcus faecalis EF-2001 ameliorates atopic dermatitis in a murine model. Nutrients 2016;8(3):636–45.
14. Bochkov V, Kadl A, Huber J, et al. Protective role of phospholipid oxidation products in endotoxin-induced tissue damage. Nature 2002;419(6902):77–81.
15. Frick VO, Rubic C, Keilholz U, et al. Chemokine/ chemokine receptor pair CCL20/CCR6 in human colorectal malignancy: An overview. World J Gastroenterol 2016;22(2):833–41.
16. Choi SC, Choi EJ, Oh HM, et al. DA-9601, a standardized extract of Artemisia Asiatica, blocks TNF-alpha-induced IL-8 and CCL20 production by inhibiting p38 kinase and NF-kappaB pathways in human gastric epithelial cells. World J Gastroenterol 2006;12(30):4850–8.
17. Shoji J, Nakanishi Y, Ishimori A, et al. Involvement of chemokines and a CD4-positive T cell subset in the development of conjunctival secondary lymphoid follicles in an atopic keratoconjunctivitis mouse model. Internat Arch Allerg Immunol 2015;167(3):147–57.
18. Kraemer WJ, Flanagan SD, Volek JS, et al. Resistance exercise induces rigion-specific adaptations in anterior pituitary gland structure and function in rats. J Appl Physiol 2013;115(11):1641–7.
19. Kim SH, Kim EK, Choi EJ. High-intensity swimming exercise increases dust mite extract and 1-chloro-2,4-dinitrobenzene-derived atopic dermatitis in BALB/c mice. Inflammation 2014;37(4):1179–85.
20. Gidlund EK, Von Walend F, Venojarvi M, et al. Human in skeletal muscle protein levels increase after resistance training in men with impaired glucose metabolism. Physiolog Rep 2016;4(23):13063.
21. Gjevestad GO, Holven KB, Ulven SM. Effects of exercise on gene expression of inflammatory markers in human peripheral blood cells: A systematic review. Curr Cardiovasc Risk Rep 2015;9(7):34.
22. Martin R, Miguel S, Chain F, et al. Faecalibacterium prausnitzii prevents physiological damages in a chronic low-grade inflammation murine model. BMC Microbiol 2015;15:67.
23. Cullen T, Thomas AW, Webb R, et al. Interleukin-6 and associated cytokine responses to an acute bout of high-intensity interval exercise: the effect of exercise intensity and volume. Appl Physiol Nutrit Metab 2016;41(8):803–8.