The Differential Impact of High-Intensity Swimming Exercise and Inflammatory Bowel Disease on IL-1β, TNF-α, and COX-2 Gene Expression in the Small Intestine and Colon in Mice Immune Activity in Swimming Exercise and IBD

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Eun-Ju Choi
Wi-Young So

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Abstract

Background and Objective: We aimed to examine the impact of high-intensity swimming exercise and inflammatory bowel disease (IBD) on IL-1β, TNF-α, and COX-2 gene expression in the small intestine and colon of mice.


Material and Methods: Forty male C57BL/6 mice were divided into 4 groups: the control group (CON), swimming exercise group (EX), 50% ethanol (EtoH) control group (50%EtoH CON), and 2,4,6-trinitrobenzene sulfonic acid group (TNBS). The EX group performed 4 weeks of exercise. Intrarectal TNBS injection induced IBD in the TNBS group; the 50%EtoH CON group received control injections. Reverse transcription and real-time polymerase chain reaction were used to examine IL-1β, TNF-α, and COX-2 mRNA expression in the small intestine and colon.


Results: IL-1β, TNF-α, and COX-2 mRNA expression was significantly increased in the EX group compared to that in the CON group (p’s<0.05). IL-1β and COX-2 mRNA expression was significantly increased in the TNBS group compared to that in the 50%EtoH CON group (p’s<0.05).


Conclusion: Thus, inflammatory cytokine IL-1β and COX-2 expression in the small intestine and colon was increased in both high-intensity swimming exercise and IBD models. However, TNF-α was increased only in the swimming exercise model. Further research is required to confirm these observations and establish swimming exercise regimes appropriate for patients with IBD.

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